Author: chemistrypubsiu

HPLC or High-performance liquid chromatography or a Highly Improved form of Column Chromatography is an analytical flexible technique in the field of analytical chemistry used for the separation of components of an organic mixture of compounds when such compounds are nonvolatile, thermally unstable, and have relatively highly molecular weights. It can be possible to analyze a sample over a vast concentration range and molecular weights by this technique. This technique was discovered by M. S. T Swett in around 1900 to study leaf pigments. It is a special type of column chromatography that pumps an analyte or sample mixtures in…